Combined detection of cyclin D1, p27 and DNA content in esophageal cancer

Objective To investigate the expressions of cyclin D1 and p27 and DNA content in esophageal cancer and adjacent normal tissues, and to discuss the relationship between them. Methods The cyclinD1 and p27 were detected by immunohistochemical staining; DNA content was measured by flow cytometry. Results The positive expression rates of cyclinD1 and p27 in cancer were 45.8% and 33.3% respectively, the DNA content in the positive group of cyclinD1 was higher than that in the negative group of cyclinD1(1.54±0.21 versus 1.08±0.43, P <0.05), while the DNA content and SPF (S-phase fraction) in the positive group of p27 were lower than those in the negative group (1.10±0.19 and 5.56%±5.18% versus 1.66±0.28 and 19.78%±6.12%, P <0.05). Conclusion The data show that the expression of cyclinD1 and p27 are related to the ontogenesis and progression of esophageal cancer. The combined detection of cyclinD1, p27 and DNA content may be indicators of diagnosis and assessment of esophageal cancer.