Birth of Lambs after Direct Transfer of Vitrified Ovine Embryos
JOURNAL OF REPRODUCTION AND DEVELOPMENT(2002)
摘要
The aim of this study was to investigate in vivo viability of vitrified ovine embryos after direct transfer using a laparoscope. In vivo-derived ovine morulae and blastocysts were equilibrated in 25% glycerol and 25% ethylene glycol solution, loaded into a heat-pulled straw and vitrified. Thirty-eight embryos were transferred into 19 recipients (2 embryos per recipient) and pregnancy was examined at 54-59 days after transfer. The pregnancy, implantation, lambing, and survival rates were 15.8% (3/19), 10.5% (4/38), 15.8% (3/19) and 7.9% (3/38), respectively. The result indicates that viable lambs can be produced by direct transfer of vitrified ovine embryos.
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关键词
vitrification,direct transfer,ewe
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