Discussion on the counting method of wine-making microbes.

In order to verify the detection function and accuracy of automatic microbial colony counter,methods of automatic and artificial colony counting were compared and analyzed using culture of different strains.The results showed that automatic colony counting was accurate and quick for the bacteria with smaller colonies,and the results were highly reproducible.The maximum error rate was found to be 3.95%,while it showed low accuracy for counting bigger colonies,such as yeasts and moulds,in such a case it showed 42.86 and 35.5% maximum error rate,respectively.It was suggested that automatic counting method should a be used in the practical application by setting up parameters reasonably,such as diameter,color,brightness and background deviation of colonies.Meanwhile,automatic counting method was also used to modified the results to improve its accuracy.