A novel method for immediate post-purification purity determination of fractions collected during high-throughput purification.
Journal of Chromatography A(2005)
摘要
Following purification, the fractions of purified samples typically are analyzed to determine the relative purities of each fraction. We report a novel technique for performing post-purification analysis immediately after each preparative LC/MS run. The Single Pass Compound Purification and Analysis System (SPACPASS) samples and stores a representative aliquot from the fraction while it is being collected. Demonstrated for ‘1:1’ fraction collections, this method of fraction purity assessment streamlined sample processing by reducing post-purification sample handling. For 97% of the collected fractions, this technique provided relative purities to within ±5% when compared with more traditional post-purification analysis.
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关键词
High throughput purification and analysis,High throughput purification,Fraction analysis,Single pass purification and analysis,HPLC/MS purification,LC/MS purification,Mass-directed fraction collection,Prep LC/MS,SPACPASS,Representative sampling,Representative flow stream sampling,Purity
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