Studies on the comparison of the constituents from Korean and Chinese Cynanchum spp. and their effects on the hyperlipidemia in rats

The constituents of C. wilfordii (CWK), C. auriculatum (CAK) in Korea and C. auriculatum (CAC) in China was compared by analysis of the TLC pattern. Their effects on the hyperlipidemia was investigated in rats induced by Triton WR-1339 and high cholesterol diet. The dried roots of CWK, CAK, CAC were extracted three times with 70% MeOH. The extract was suspended in water and then partitioned with chloroform and concentrated. The extracts (50mg/kg, 200mg/kg) were orally administrated every day for 7 days. In the hyperlipidemic rats induced by Triton WR-1339, CWK and CAK significantly decreased total cholesterol, triglyceride, otherwise significantly increased HDL. CWK and CAK significantly decreased AST and ALT. Also, in the hyperlipidemic rats induced by high cholesterol diet, CWK, CAK and CAC showed significant decreasing effects on total cholesterol, and especially CWK increased HDL and significantly decreased LDL. Korean CAK showed increasing effects on HDL. These results indicated that TLC analysis showed similar patterns between CWK, CAK and CAC, so the constituents of CWK and CAK is similar. CWK and CAK CAC were all effective in the hyperlipidemic rats induced by Triton WR-1339 and high cholesterol diet.