Birth after double cryopreservation of human oocytes at metaphase II and pronuclear stages.

To document the possibility to double-cryopreserve human metaphase II and pronuclear oocytes.Case report.University-based IVF center.A couple with primary andrological infertility.Pronuclear stage oocytes derived after sperm injection into frozen-thawed metaphase II oocytes were cryopreserved again for later transfer.Post-thaw survival rates and pregnancy.Five metaphase II oocytes survived the freeze-thaw procedure. Three of five oocytes presented with two pronuclei after sperm injection and were cryopreserved again. Post-thawing, all three oocytes developed into good quality embryos for transfer. A singleton pregnancy was achieved, resulting in the birth of a healthy girl.Double-cryopreservation of oocytes at metaphase II and at pronuclear stage is a treatment option in human-assisted reproduction.