Mannheimia (Pasteurella) Haemolytica Leukotoxin Binding Domain Lies Within Amino Acids 1 To 291 Of Bovine Cd18

Previously, we identified bovine CD18 as the receptor for leukotoxin secreted by Mannheimia (Pasteurella) haemolytica. In this study, we constructed bovine-murine CD18 chimeras to locate the leukotoxin binding domain on CD18. Leukotoxin specifically lysed transfectants expressing bovine CD18 fragment encompassing amino acids 1 to 291, indicating that leukotoxin binding domain lies within amino acids I to 291 of bovine CD18.