Protection of cardiomyocytes from coxsackievirus B3 by sophocarpine

AIM:To observe the effect of sophocarpine(SC)on the myocarditis model established by coxsackievirus B3(CVB3)in cardiomyocytes from neonatal rats.METHODS:Cardiomyocytes from neonatal rats were divided into four groups:infected group,which was infected by CVB3;SC treated group,which was incubated with SC(100 mg·L~(-1))after viral infection;SC control group,which was incubated with SC(100 mg·L~(-1))only;and cell control group.The cytopathic effect(CPE),beating frequency of myocardial cells and the lactate dehydrogenase(LDH)levels in supernatant was tested at day 2,3,5 respectively after the treatment. The cultured myocytes were incubated with different concentrations of SC(12.5-400 mg·L~(1))after infected by CVB3.The CPE and the levels of LDH were tested at day 2,3,5 after treatment.RESULTS:(1)SC had pro- tective effect on CVB3-infected cardiomyocytes.The cytopathic effect was lighter and the LDH level was lower in SC treated group(100 mg·L~(-1))than that in infected group.(2)SC in concentration between 12.5-300mg·L~(-1) could protect the eardiomyocytes from CVB3;it improved CPE and decreased the LDH levels.While in a higher concentration(400 mg·L~(-1)),SC exacerbated the CPE and increased LDH levels.CONCLUSION:SC in certain concentration could protect eardiomyocytes from CVB3 in vitro.