Effect of 2',4'-Dihydroxy-4',6'-Dimethoxychalcone on IL-8 Expression in Lung Adenocarcinoma-associated Cells

There are three major groups in lung adenocarcinoma tissue,including tumor-associated macrophage,high invasion tumor cell and low risk of invasion cell.This study was conducted to investigate the effects of 2′,4′-dihydroxy-4′,6′-dimethoxychalcone(chalcone) on IL-8 expression in lung adenocarcinoma.We focused on the influence of secretion and expression of IL-8 in the preceding three kinds of cells treated with the single compound mentioned above.The methods such as quantitative real-time PCR,ELISA,Dual-Luciferase Reporter Assay System were used to detect the volume of expressed IL-8 and the Electrophoretic Mobility Shift Assay was employed to assay the upstream regulatory mechanism.The series of analysis indicated that the single compound was able to inhibit secretion of IL-8 in CL1-0,with the inhibition effect of 50%.Conversely,both of upstream regulatory factors of IL-8 gene called Active Protein-1(AP-1),NF-κB which the DNA-binding active were enhanced after treating the single compound in conditioned medium-stimulated high risk of invasion cell line CL1-5,and their enhancing effect was about 30%.The similar phenomenon emerged in tumor-associated macrophages,with the inhibition effect of 24.9%.The result indicated that chalcone has a positive effect on inhibiting IL-8 expression during early-stage of lung adenocarcinama progress.