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桃‘豫农矮砧1号’快繁体系初代培养基的初探

Chinese Agricultural Science Bulletin(2013)

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Abstract
通过对不同完全培养基的筛选,进而探寻能够提高桃‘豫农矮砧1号’萌芽率的初代培养基,为桃‘豫农矮砧1号’快繁体系的建立打下坚实的基础,以桃‘豫农矮砧1号’的茎段为外植体,进行离体培养中初代培养基的筛选,以MS为基本培养基,附加不同浓度生长素IBA(3-吲哚丁酸)与细胞分裂素6-BA(6-苄基腺嘌呤)。结果表明:桃‘豫农矮砧1号’快繁体系中最佳初代培养基为MS+1.6mg/L6-BA+0.4mg/LIBA,芽诱导率在IBA 0.1~1.0mg/L,6-BA 0.8~1.6mg/L的浓度范围内随激素浓度的上升而上升,并且以MS+1.6mg/L6-BA+0.4mg/LIBA的芽诱导率最高。
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Key words
peach,initial culture,fast reproduction,rootstock
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