槲皮素-罗丹明6G荧光猝灭体系研究

Shandong Chemical Industry(2012)

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Abstract
以Stern-Volmer方程和罗丹明6G吸收光谱研究了槲皮素-罗丹明6G荧光猝灭体系。结果表明,槲皮素、罗丹明6G间发生静态猝灭,生成稳定复合物,二者的结合常数和结合点数为k1=2.63×106L·mol-1,n1=1.14;k2=7.42×105L·mol-1,n2=1.07。槲皮素的质量浓度在0.01~0.2μg·mL-1和0.2~5.0μg·mL-1范围内与F0/F成正比,检出限为5.9ng·mL-1,加标回收率为91.7%~104%,方法可用于槲皮素含量测定。
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Key words
rhodamine 6G,quercetin,fluorescence quenching,mechanism
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