昆明山海棠总生物碱对人结肠癌HCT116细胞增殖和凋亡的影响
Acta Academiae Medicinae Militaris Tertiae(2009)
Abstract
目的研究昆明山海棠总生物碱(total alkaloids of tripterygium hypoglaucum hutch,THHta)对人结肠癌HCT116细胞增殖及凋亡的影响。方法采用MTT法检测THHta对HCT116细胞增殖的抑制作用;用倒置及荧光显微镜观察THHta作用后HCT116细胞形态的变化;流式细胞术检测THHta对HCT116细胞周期及凋亡的影响,Western bolt检测凋亡相关caspase-3及PARP蛋白表达。结果THHta能显著抑制HCT116细胞增殖,抑制率与剂量和时间呈依赖关系。THHta作用HCT116细胞48、72h的IC50值小于20μg/ml。THHta(10μg/ml)能诱导HCT116细胞G0/G1期比例增加及细胞凋亡,caspase-3活化及PARP蛋白剪切显著增强。结论THHta能显著抑制HCT116细胞增殖,并可诱导HCT116细胞G0/G1期阻滞及细胞凋亡。
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Key words
HCT116 cell,proliferation,apoptosis,alkaloids,tripterygium hypoglaucum hutch
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