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浙贝母总生物碱提取工艺优化研究

World Science and Technology(Modernization of Traditional Chinese Medicine and Materia Medica)(2012)

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Abstract
目的:优化浙贝母中总生物碱提取工艺。方法:以总生物碱含量为指标,采用酸性染料比色法测定浙贝母总生物碱含量,以乙醇用量、乙醇浓度、回流时间为主要影响因素开展正交试验,以回流次数单因素考察优化提取工艺。结果:最佳提取工艺为70%乙醇,6倍量溶媒,提取2次,每次2 h。结论:所优选出的提取工艺方法合理可行,稳定可靠。
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Key words
total alkaloids,Bulbus Fritillariae Thunbergii,extraction technology,orthogonal design,acid dye colorimetry method
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