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中药鳖甲提取物抗肝纤维化的实验研究

Yinping TANG,Laying XU,Yanwen LIU

Journal of Hubei University of Chinese Medicine（2011）

Cited 9|Views11
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Abstract
目的 探讨中药鳖甲提取物的抗肝纤维化活性及作用机制.方法 传代培养的大鼠肝星状细胞(HSC-T6)与从鳖甲中提取分离得到的小组分Dj60-Bj616各浓度组共同培养72 h后,采用酶联免疫吸附剂测定法(ELISA)测定各浓度组Ⅳ型胶原(ColⅣ)和Ⅲ型胶原(ColⅢ)的含量,同时设空白对照组和刺激对照组.结果 鳖甲小组分Bj611-Bj613的各浓度组的Ⅳ型胶原和Ⅲ型胶原的含量与空白对照组、刺激对照组比较均存在显著性差异(P<0.05).结论 鳖甲提取物通过降低Ⅳ型胶原和Ⅲ型胶原的含量而发挥抗肝纤维化的作用.
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extract of Carapax Trionycis,ColⅢ,ColⅣ,ELISA,anti-hepatic fibrosis activity
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