两株脂肪类混合细菌拆分缩水甘油丁酸酯的工艺研究
Journal of Zhengzhou University(Engineering Science)(2012)
Abstract
由于从土壤筛选出十几个单株细菌拆分效果不理想,从中优选出两株脂肪类细菌混合使用,拆分效果良好.其选择性拆分外消旋体缩水甘油丁酸酯的工艺为:淀粉为碳源,牛肉膏为氮源,初始pH=8.0,温度30℃,转速200 r/min,摇瓶2 d,产酶量为1.5 U.在最适条件下培养两株细菌拆分反应,并考察工艺条件对拆分反应效果的影响.结果表明,工艺条件在温度28℃,pH=6.0,30 mL脂肪类双菌液中加入0.5 g缩水甘油丁酸酯,在200 r/min下振荡12 h,拆分效果良好,(R)-缩水甘油丁酸酯的对映体过量值(ee)为92.8%,转化率为83.2%.
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Key words
alkaline lipase,optical resolution,glycidyl butyrate
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