油菜蜂花粉中游离氨基酸含量的测定
Heilongjiang Agricultural Sciences(2012)
Abstract
用双光束紫外分光光度计测定油菜蜂花粉中游离氨基酸的含量。在该过程中,花粉用43°青稞白酒提取,提取液先用氢氧化钡沉淀除去色素和蛋白质,再用水合茚三酮显色,通过双光束紫外分光光度法测氨基酸含量。结果表明:在相同质量的花粉中,未破壁油菜花粉中游离氨基酸的含量为1.87%~4.91%,破壁花粉中游离氨基酸的含量为2.46%~8.26%。该方法中的仪器易于操作,测定结果准确可靠。
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Key words
double beam UV spectrophotometer,amino acids,bee pollen
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