‘Red pigment’ from ADE-2 mutants of S. cerevisiae prevents DNA cleavage by restriction endonucleases
FEBS Letters(1985)
Abstract
Protection of DNA from cleavage by restriction endonucleases EcoRI, HindIII, BamHI, and Bg/II with red pigment, produced by ADE-2 mutants of Saccharomyces cerevisiae is demonstrated. Purification of yeast DNA from pigment can be achieved by chromatography on hydroxyapatite columns.
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Key words
ADE-2 locus,Red pigment,Restriction endonuclease,(S. cerevisiae)
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