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双重胶体金层析法快速检测番茄环斑病毒和烟草环斑病毒RT-PCR扩增子

Chinese Agricultural Science Bulletin(2011)

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Abstract
番茄环斑病毒和烟草环斑病毒是中国进境检疫性有害生物,此研究建立了单个和双重胶体金层析快速检测方法,在15min即可获得对双标记PCR产物的检测结果。单个胶体金层析是在一张层析膜上点上荧光素(或地高辛)的单克隆抗体作为T检测点,经生物素-荧光素(或生物素-地高辛)双标记的RT-PCR扩增产物可在T点上被检测到。双重胶体金层析是在一张层析膜上分别点上荧光素和地高辛的单克隆抗体作为T1和T2检测点,经生物素-荧光素和生物素-地高辛双标记的两种病毒的RT-PCR扩增产物可分别在T1和T2点上被检测到。
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Key words
colloidal gold,duplex detection,ToRSV,RT-PCR,TRSV
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