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抗非洲猪瘟病毒VP73蛋白单克隆抗体的制备

Progress in Veterinary Medicine(2012)

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Abstract
为了制备抗非洲猪瘟病毒(ASFV)VP73蛋白的单克隆抗体(MAb),原核表达并纯化了该病毒的重组VP73蛋白,按常规方法免疫Balb/c小鼠,取其脾细胞与SP2/0骨髓瘤细胞融合,对杂交瘤细胞进行筛选,阳性孔经3次有限稀释法亚克隆,最终获得了一株稳定分泌抗ASFV VP73蛋白MAb的杂交瘤细胞株,命名为1C8。ELISA检测和Western blot分析表明,所获得的MAb与重组VP73蛋白呈阳性反应,腹水效价为1.024×105,该MAb的抗体类型为IgG1亚类。
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Key words
VP73 protein,monoclonal antibody,African swine fever virus
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