两种测定方法对微生物产酶菌株酶活测定的比较
wf(2011)
Abstract
利用紫外分光光度法和福林酚法对微生物产蛋白酶菌株进行酶活活性的测定。采用酪蛋白琼脂培养基对稀释分离的样品进行初筛,选取水解圈直径与菌落直径比值较大的菌株进行活性测定。结果发现,紫外光度法和福林酚法对酶活测定结果相差不大,紫外光度法因操作简捷,所用试剂少等优点,更加适用于企业用酶活性的测定。
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Key words
Folin method,enzyme activity,protease,UV spectrophotometry
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