Cloning and Bioinformatics Analysis of an Endoglucanase 2 from Trichoderma reesei

Yanling Yang,Lihua Liu,Miao Diao,Zhiwei Lin,Wunian Guo,Shihua Wang

Software Engineering, 2009. WCSE '09. WRI World Congress（2009）

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摘要

Trichoderma reesei CBS368, a strong endoglucanase 2 (EG 2) producing strain, was isolated in our laboratory. The DNA fragment encoding the EG 2 was cloned in pMD18-T and sequenced. In order to achieve its expression, the vector pET28a (+) and pET32a (+) were used as expression vector, and the recombinant EG 2 was successfully expressed in E. coli BL21 (DE3). Bioinformatic analysis was conducted using some online or offline services.

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关键词

strong endoglucanase,endoglucanase,eg 2 strain production,cellular biophysics,bioinformatic analysis,vector pmd18-t,dna fragment encoding,vector pet28a,microorganisms,encoding,online service,sequences,sequence,expression,trichoderma reesei cbs368,e. coli,clone,expression vector,offline service,recombinant eg,cloning analysis,dna,dna fragment,bioinformatics,bioinformatics analysis,signal processing,proteins,biochemistry,dna fragmentation,cloning,amino acids,capacitive sensors,chemical analysis

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