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职业迁徙
个人简介
I have a long term experience in molecular and cellular pharmacology of antitumor agents, optimization of therapeutic strategies with cytotoxic drugs, identification and validation of novel therapeutic targets. Recently, I have investigated the role of non-coding RNAs, mostly miRNAs, in cancer development, progression and as novel targets for cancer therapy. In this context, I acquired good expertise in a number of approaches for the functional characterization of genes, including their isolation and detection, analysis of gene expression profiles, miRNA target prediction and validation. During the past two years, I have processed 30 soft tissue sarcoma and three diffuse malignant peritoneal mesothelioma specimens obtained by a surgical resection in attempts to establish long-term cell lines and xenografts PDX. I have competences in cellular pharmacology techniques including maintenance of in vitro cell cultures, cytotoxicity and apoptosis assays; stable and transient transfections to modulate the expression of genes and miRNAs; extraction of nucleic acids, preparation of cellular protein lysates from cell lines and tumors explanted from animal models; Western Blotting; immunoprecipitation techniques; quantitative PCR and RT-PCR; isolation of metastatic tumor cells in lung of xenograft mouse; immunofluorescence techniques and ELISA; migration and invasion assay.
研究兴趣
论文共 93 篇作者统计合作学者相似作者
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bioRxiv : the preprint server for biology (2024)
Molecular Cancer Therapeuticsno. 12_Supplement (2023)
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作者统计
#Papers: 90
#Citation: 2764
H-Index: 30
G-Index: 50
Sociability: 6
Diversity: 0
Activity: 0
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