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Among patients with AIDS, leukemia or other cancers, organ or bone marrow transplants, and similar immunocompromising risk factors, the incidence of opportunistic mycoses and the number of different fungal pathogens are increasing dramatically. For many of these fungi, the definition of a species and the recognition of pathogen are highly problematic. Conventional methods of identification are based on morphological and physiological characteristics and are often time-consuming, difficult to interpret, and inconsistent. This laboratory is using DNA-based methods to (i) identify fungal pathogens, (ii) resolve taxonomic issues, (iii) facilitate epidemiological studies, (iv) recognize strains with clinically relevant phenotypes, such as resistance to antifungal drugs, (v) elucidate the origin(s) of diversity and the population genetics of the major pathogens, and (vi) explore functional genomics to identify virulence factors. We have developed reliable methods to genotype strains and are analyzing gene sequences to clarify the phylogeny of controversial taxa.
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European Journal of Medical and Health Sciencesno. 4 (2020)
European Journal of Medical and Health Sciencesno. 4 (2020)
Jessica Pérez-Alquicira,Esther van der Knaap,Kristin Mercer,Leah McHale,Thomas Mitchell,Jose Luna-Ruiz, Esther Texocotitla-Vázquez,Ofelia Vargas-Ponce,Andy Michel
ASM Press eBookspp.235-259, (2014)
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