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Research interests of the Lunte group include: (1) microanalytical methods for the investigation of the transport and metabolism of peptides across the blood-brain barrier (2) separation-based sensors employing on-line microdialysis coupled to microchip electrophoresis (3) cell-based assays on chips, and (4) microchip-based diagnostics for cardiovascular and metabolic diseases.
Microanalytical methods for the investigation of the transport and metabolism of peptides across the blood-brain barrier: An insight into peptide transport and metabolism is important for effective drug design and the understanding of neurological disorders. Crucial to these studies is the development of analytical methodologies that are capable of monitoring these biologically important compounds at physiologically relevant concentrations. The particular analytes of interest include neuroactive peptides, amino acids and catecholamines. Release, transport, and metabolism of these substances can be investigated in vitro using a cell culture model or in vivo using microdialysis sampling. Due to the small sample volumes generated by these methods, microcolumn-based separation methods have been employed for analysis. These include capillary and microchip electrophoresis and microcolumn liquid chromatography. To obtain the requisite sensitivity for these assays, laser-induced fluorescence and electrochemical detection and mass spectrometric methods have been employed. The focus of this research has been primarily on substance P and dynorphin. These two neuropeptides have been shown to be important in neuropathic and chronic pain as well as depression.
Microanalytical methods for the investigation of the transport and metabolism of peptides across the blood-brain barrier: An insight into peptide transport and metabolism is important for effective drug design and the understanding of neurological disorders. Crucial to these studies is the development of analytical methodologies that are capable of monitoring these biologically important compounds at physiologically relevant concentrations. The particular analytes of interest include neuroactive peptides, amino acids and catecholamines. Release, transport, and metabolism of these substances can be investigated in vitro using a cell culture model or in vivo using microdialysis sampling. Due to the small sample volumes generated by these methods, microcolumn-based separation methods have been employed for analysis. These include capillary and microchip electrophoresis and microcolumn liquid chromatography. To obtain the requisite sensitivity for these assays, laser-induced fluorescence and electrochemical detection and mass spectrometric methods have been employed. The focus of this research has been primarily on substance P and dynorphin. These two neuropeptides have been shown to be important in neuropathic and chronic pain as well as depression.
Research Interests
Papers共 208 篇Author StatisticsCo-AuthorSimilar Experts
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ELECTROPHORESIS (2024)
CELLSno. 22 (2023): 2592-2592
ELECTROANALYSISno. 12 (2022): 1913-1927
Tools and Trends in Bioanalytical Chemistry (2021)
The Analystno. 5 (2020): 1768-1776
MICROMACHINESno. 6 (2020): 593-593
Analytical and bioanalytical chemistryno. 24 (2020): 6101-6119
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