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Professor Rokita’s research program is united by a common interest in describing the structure and activity of biological macromolecules through their essential chemical reactivity. The methods of organic synthesis, physical organic chemistry, protein and nucleic acid chemistry, biochemistry and molecular biology are applied to questions on enzyme catalysis and nucleic acid modification. Current projects include enzymatic dehalogenation and reversible covalent chemistry expressed by quinone methide intermediates.
Reductive dehalogenation in nature. Organohalides are distributed widely in the environment and originate from both biological and industrial sources. Under aerobic conditions, these compounds are typically consumed by oxidative and hydrolytic metabolism. However, vertebrates also express the unusual ability to promote reductive dehalogenation of natural iodotyrosine derivatives related to the thyroid hormone thyroxine. Iodide recovery from these compounds is promoted by iodotyrosine deiodinase, an enzyme crucial for human health. This same enzyme has also been discovered in all metazoa sequenced to date as well as some bacteria. The role of reductive dehalogenation in these additional organisms is not yet clear. Investigations are currently designed to identify the origins of this enzyme, characterize its biological role in non-vertebrates and understand its catalytic mechanism that includes a role of its bound flavin. Flavin-dependent reactions are associated with a wide variety of metabolic transformations but its use in reductive dehalogenation is quite rare. Results of this research will help to expand the known repertoire of flavin chemistry and will guide its future use in bioremediation of organohalide pollutants.
Professor Rokita’s research program is united by a common interest in describing the structure and activity of biological macromolecules through their essential chemical reactivity. The methods of organic synthesis, physical organic chemistry, protein and nucleic acid chemistry, biochemistry and molecular biology are applied to questions on enzyme catalysis and nucleic acid modification. Current projects include enzymatic dehalogenation and reversible covalent chemistry expressed by quinone methide intermediates.
Reductive dehalogenation in nature. Organohalides are distributed widely in the environment and originate from both biological and industrial sources. Under aerobic conditions, these compounds are typically consumed by oxidative and hydrolytic metabolism. However, vertebrates also express the unusual ability to promote reductive dehalogenation of natural iodotyrosine derivatives related to the thyroid hormone thyroxine. Iodide recovery from these compounds is promoted by iodotyrosine deiodinase, an enzyme crucial for human health. This same enzyme has also been discovered in all metazoa sequenced to date as well as some bacteria. The role of reductive dehalogenation in these additional organisms is not yet clear. Investigations are currently designed to identify the origins of this enzyme, characterize its biological role in non-vertebrates and understand its catalytic mechanism that includes a role of its bound flavin. Flavin-dependent reactions are associated with a wide variety of metabolic transformations but its use in reductive dehalogenation is quite rare. Results of this research will help to expand the known repertoire of flavin chemistry and will guide its future use in bioremediation of organohalide pollutants.
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Proceedings of the National Academy of Sciences of the United States of Americano. 21 (2024): e2322501121-e2322501121
NUCLEIC ACIDS RESEARCHno. 11 (2023): 5341-5350
Blessing D Deeyaa,Steven E Rokita
Protein science : a publication of the Protein Societyno. 1 (2018): 68-78
semanticscholar(2017)
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