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个人简介
Our laboratory specializes in two areas, infectious diseases, particularly
viral diseases, and ultrastructure-function relationships. Electron
microscopy (EM) is the focus of the investigative techniques and includes
preparative methods such as negative staining, thin sectioning,
ultracryomicrotomy and immunolabeling of acrylic and frozen sections.
We are especially interested in methods for diagnosing viral illnesses by
EM, and are involved in developing better, more sensitive and faster,
methods for detection. While molecular techniques for detecting organisms
are very sensitive, they all require specific reagents, and if the correct
probe is not determined a priori, the test is negative. EM offers an open
view of any viruses or unsuspected organisms that may be present. We make
use of concentration and enhancement methods to increase the chances of
detecting viral agents in fluid specimens. Additionally, we have
described a method for selecting small focal areas of pathology in tissue
by confocal microscopy to be embedded and examined by EM, increasing the
chances of visualizing organisms. Infectious diseases are the leading
cause of death worldwide and the third leading cause in the US. With
advanced therapies for cancer patients and many patients living longer
with their disease, a whole new population of infectious
disease-susceptible patients has emerged. Chemotherapy, radiation, and
bone marrow transplantation are permitting longer survival, but cause
immunosuppression and consequently, strange, unusual diseases, such as
polyomavirus infections, sometimes in uncommon body sites. We work
closely with physicians to detect and monitor the clearance of
polyomavirus infections in bone marrow and kidney transplant patients. We
detect food-borne outbreaks on campus, and we test numerous specimens from
patients with infectious diseases. We also serve on the Duke Biodefense
Team due to our capability to detect and differentiate poxvirus infections
from those of herpesvirus infections rapidly (within minutes).
viral diseases, and ultrastructure-function relationships. Electron
microscopy (EM) is the focus of the investigative techniques and includes
preparative methods such as negative staining, thin sectioning,
ultracryomicrotomy and immunolabeling of acrylic and frozen sections.
We are especially interested in methods for diagnosing viral illnesses by
EM, and are involved in developing better, more sensitive and faster,
methods for detection. While molecular techniques for detecting organisms
are very sensitive, they all require specific reagents, and if the correct
probe is not determined a priori, the test is negative. EM offers an open
view of any viruses or unsuspected organisms that may be present. We make
use of concentration and enhancement methods to increase the chances of
detecting viral agents in fluid specimens. Additionally, we have
described a method for selecting small focal areas of pathology in tissue
by confocal microscopy to be embedded and examined by EM, increasing the
chances of visualizing organisms. Infectious diseases are the leading
cause of death worldwide and the third leading cause in the US. With
advanced therapies for cancer patients and many patients living longer
with their disease, a whole new population of infectious
disease-susceptible patients has emerged. Chemotherapy, radiation, and
bone marrow transplantation are permitting longer survival, but cause
immunosuppression and consequently, strange, unusual diseases, such as
polyomavirus infections, sometimes in uncommon body sites. We work
closely with physicians to detect and monitor the clearance of
polyomavirus infections in bone marrow and kidney transplant patients. We
detect food-borne outbreaks on campus, and we test numerous specimens from
patients with infectious diseases. We also serve on the Duke Biodefense
Team due to our capability to detect and differentiate poxvirus infections
from those of herpesvirus infections rapidly (within minutes).
研究兴趣
论文共 44 篇作者统计合作学者相似作者
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Somenath Datta,Brett M. Antonio,Nathan H. Zahler, Jonathan W. Theile, Doug Krafte,Hengtao Zhang,Paul B. Rosenberg,Alec B. Chaves,Deborah M. Muoio,Guofang Zhang,Daniel Silas,Guojie Li,
JOURNAL OF CLINICAL INVESTIGATIONno. 5 (2024)
Anita E. Grootemaat, Niek Wiersma,Sanne van der Niet, Irene M. Schimmel,Sandrine Florquin,Eric A. Reits,Sara E. Miller,Nicole N. van der Wel
Microbiology spectrumno. 6 (2023): e0302923-e0302923
Zhiyong Liu,Arpine Sokratian,Addison M. Duda,Enquan Xu,Christina Stanhope, Amber Fu, Samuel Strader,Huizhong Li, Yuan Yuan,Benjamin G. Bobay,Joana Sipe,Ketty Bai,
SCIENCE ADVANCESno. 46 (2023)
Zhiyong Liu,Arpine Sokratian,Addison M Duda,Enquan Xu,Christina Stanhope, Amber Fu, Samuel Strader,Huizhong Li, Yuan Yuan,Benjamin G Bobay,Joana Sipe,Ketty Bai,
Research squareno. 46 (2023): eadi8716-eadi8716
MICROSCOPY RESEARCH AND TECHNIQUEno. 7 (2022): 2740-2747
ASAIO journal (American Society for Artificial Internal Organs : 1992)no. 10 (2021): 1087-1096
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