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个人简介
Key discoveries as PhD student
• Discovery of membrane lipid sorting at the trans Golgi network.
• Determined the first eukaryotic, comprehensive membrane lipidome of purified sub-cellular organelles in absolut quantitation.
• First isolation of post Golgi secretory vesicles from living cells.
• First genome wide visual screen for sorting factors in eukaryotic membrane traffic.
Key discoveries as postdoctoral fellow:
• The molecular mechanism of endoplasmic reticulum (ER) membrane fusion in metazoans and yeast is SNARE independent and depends on the dynamin GTPases called Atlastin in metazoans and Sey1p in yeast or Rhd3 in plants.
• Reconstitution of ER membrane fusion from purified components.
• Stacks of ER membrane sheets are connected by helicoidal membrane ramps.
• Discovery of the functional importance of ER membrane fusion for the homeostasis of neutral lipid metabolism in higher eukaryotes and the size regulation of fat storing organelles called lipid droplets.
Current research direction in the Klemm Laboratory
The functional coupling of the endoplasmic reticulum and mitochondria with lipid droplets in fat storing cells called adipocytes.
Extended Biosketch on Robin's work:
Robin did his undergraduate work in Biochemistry at the Goethe University in Frankfurt, Germany. For his masters/diploma work he focused on the purification and characterization of a eukaryotic ABC transporter.
He then moved on to do his PhD in the lab of Kai Simons at the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden. He worked on the mechanisms of cargo sorting at the trans Golgi network. Robin carried out a genome wide visual screen in yeast and developed affinity isolation methods for the purification of secretory vesicles and other organelles from living cells. He was involved in developing quantitative mass spectrometry based lipidomics to assess the lipid composition of the purified organelles and found that the Golgi apparatus not only sorts proteins but also membrane lipids. In particular sterols and sphingolipids are integral part of the machinery that produces secretory vesicles.
As a postdoc Robin worked on mechanisms that generate organelle shape in the lab of Tom Rapoport at Harvard Medical School. He reconstituted a new intracellular membrane fusion pathway connecting the endoplasmic reticulum into a functional network of tubules and membrane sheets. Together with crystal structures of the fusion protein representing snap shots of the fusion reaction Robin's work suggested a new mechanism of homotypic ER fusion.
The Klemm Lab is focused on understanding the mechanisms that functionally couple lipid droplets biogenesis to the ER and mitochondria.
研究兴趣
论文共 30 篇作者统计合作学者相似作者
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Cell Reportsno. 4 (2024): 114093-114093
FEBS lettersno. 10 (2024): 1274-1291
Cell reportsno. 5 (2021): 108710-108710
Contact (2021): 2515256421993708-2515256421993708
Seminars in cell & developmental biology (2020): 1-3
bioRxiv (Cold Spring Harbor Laboratory) (2020)
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作者统计
#Papers: 30
#Citation: 3287
H-Index: 20
G-Index: 25
Sociability: 5
Diversity: 2
Activity: 13
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