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个人简介
We are working on the development of new analysis technologies that will advance genome sequencing, polymorphism characterization, proteomics and health care diagnostics. Toward this end we developed the first Capillary Array Electrophoresis instruments and the energy transfer dye label concepts that were used in the sequencing of the Human Genome. Our approach is to exploit miniaturization of chemical and biochemical analysis along with high-sensitivity fluorescence detection to produce integrated microfabricated lab-on-a-chip microanalysis systems. We are now going beyond the traditional lab-on-a-chip concept to the development of chemical and biochemical microprocessors. We have developed microphotolithographic methods for fabricating capillary electrophoresis systems on glass substrates or chips and the development of methods for DNA sample preparation on these devices. The advantages of these devices are that very small amounts of sample are needed, the electrophoretic separations are 10-100 times faster than conventional techniques, and miniaturization permits the fabrication of massively parallel analysis devices. Photolithographic CE systems have been fabricated and used to perform high speed DNA fragment analyses, PCR fragment sizing and DNA sequencing. Methods have also been developed for fabricating CAE chips with from 96 to 384 capillaries that are capable of analyzing large numbers of samples in parallel. We have also developed methods for integrating PCR-based sample preparation on these chips to make the first miniaturized DNA analysis bioprocessors. Future work will focus on the fabrication and operation of more complex DNA sample preparation and analysis systems including valves, vents, reactors, pumps etc. to perform nanoliter chemistries that would not be feasible or practical in a conventional macroscopic laboratory. Examples include the development of chips to evolve selected RNA populations automatically, the development of ultra-high gain cascaded PCR reactors, chips to perform analysis of single cell genetics and expression, the development of chip-based logic circuits using DNA as the information carrier in microfluidic channel "wires", the development of microchemical and biochemical analysis devices to explore for chemical signs of extraterrestrial life, and the development of portable clinical health care monitors.
研究兴趣
论文共 721 篇作者统计合作学者相似作者
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LAB ON A CHIPno. 9 (2024): 2551-2560
Research Square (Research Square) (2023)
The Planetary Science Journalno. 4 (2021)
Bulletin of the AASVol. 53, Issue 4 (Planetary/Astrobiology Decadal Survey Whitepapers)no. 4 (2021)
Bulletin of the AASVol. 53, Issue 4 (Planetary/Astrobiology Decadal Survey Whitepapers)no. 4 (2021)
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