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职业迁徙
个人简介
Research
Methods for structural biology
Developing improved methods for determining structures of proteins and other macromolecules.
For decades, crystallography has been the primary method for determining the three-dimensional structure of a protein, which provides an essential framework for a detailed understanding of its biochemistry. We have been focusing on extending the scope and power of the methods used in protein crystallography. With the recent revolution in the ability of cryo-electron microscopy methods to determine structure at high resolution, we are now also looking at the intersection between these techniques.
In crystallographic theory, we focus on the understanding of probability distributions relating the structure factors that arise from a diffraction experiment. A detailed understanding of these probability distributions underlies new developments in maximum likelihood methods, which we are implementing in our program Phaser. The current version of Phaser can solve structures by molecular replacement (that is, using the known structures of related proteins), by using the information from single-wavelength anomalous diffraction (SAD), and by a combination of the two. By accounting better for the effects of errors, these new methods can cope with trends to collect data from poorer crystals to higher resolution, and can solve structures that evaded earlier approaches.
Cryo-electron microscopy shares some of the theoretical underpinnings of crystallography, since both methods involve analyses in Fourier space. As a result, we can now explore ways in which the lessons we learned in implementing likelihood methods for crystallography can be transposed to the new methods in cryo-EM.
研究兴趣
论文共 8 篇作者统计合作学者相似作者
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mag(2007)
引用203浏览0引用
203
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Glen D Edmonton Armstrong, S Cockle,Bart Hazes, Michel H Willowdale Klein, Sheena Aurora Loosmore,Raymond P Oomen,Randy J Edmonton Read,Penelope E Stein
mag(1994)
引用23浏览0引用
23
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