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Our group has been working on industrial enzymes like protease, lipase, amylase and chitinase since past thirty years. Current research is focused on keratinase, lipase and gamma-glutamyl transpeptidase. Our laboratory is continuously engaged in isolation and identification of bacterial and yeast isolates from selective habitats through enrichment methods to select potential enzyme producers. We work towards strain improvement by taking a protein to gene approach involving the methods of cloning, heterologous expression and developing a variety of vector-host combinations. This is followed by biochemical and biophysical studies of the proteins. In-silico structural and functional analysis including homology modeling, docking, etc. is conducted to solve the protein structure. Site directed mutagenesis is often employed to validate the in-silico analysis. Another key area is protein engineering to obtain catalytically efficient enzymes. We also work on scale-up of the proteins and their industrial applications.
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Papers共 276 篇Author StatisticsCo-AuthorSimilar Experts
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Catalysis Researchno. 03 (2022): 1-69
Fundamentals of Bacterial Physiology and Metabolismpp.431-464, (2021)
Fundamentals of Bacterial Physiology and Metabolismpp.209-233, (2021)
Fundamentals of Bacterial Physiology and Metabolismpp.81-98, (2021)
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