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The Facility, in which the two executives in service participate, studies the molecular regulation of messenger RNA metabolism and the function of non-coding RNA in mammal cells. The aim of our work is to understand how these processes can affect the pathogeneases of human diseases of wide social importance such as inflammation and cancer. We follow two main widely intersected lines of research:
Regulation of degradation of unstable transcribeds
The speed at which a messenger RNA (mRNA) is degraded is as important as the speed at which it is transcribed. Transcription and degradation determine the amount of each mRNA at any given time. Since the mRNAs coding for regulatory proteins (transcription factors, cyclones, oncogenes, cytokines, chemokines, etc.) are largely unstable, it is essential to understand the molecular mechanisms that a cell implements to regulate the rate of degradation of each mRNA in response to the variety of stimuli to which it is subjected during its lifetime. It is also known that transcription and degradation speeds are intimately connected. A key event in the degradation of an mRNA is the interaction of RNA-binding proteins (RBP) with specific sequences located in the un transcribed 3' (3' UTR) stroke of the transcribed. Once linked, these proteins recruit exoribonucleolytic enzymes responsible for mRNA degradation. The interaction with RNA and the functionality of RBPs is regulated by signaling pathways that convert the stimuli to which each cell is subjected in specific biochemical modifications of the RBS themselves. In addition, our laboratory recently proved that the interaction of a non-coding RNA (ncRNA, see point 2) with an RBP affects the pro-degrading function of the latter.
Regulation of gene expression by non-coding RNAs Only
a small part of the human genome (< 2%) coding for proteins. The remaining part, while not giving rise to proteins, does not constitute useless "junk", but can be transcribed and generate non-coding RNA (ncRNAs) that perform an important regulatory function on the expression of other genes. Hence the objective of identifying possible changes in expression or function at the expense of ncRNA in the course of diseases such as cancer. NCRNAs include both very short molecules (20-23 nucleotides, microRNAs [miRNAs]) and longer molecules (over 200 nucleotides, lncRNA). In the case of miRNAs, their pathogenic role in many human diseases is proven. In the case of lncRNAs, experimental research can be considered in the early days although it has recently been shown that some lncRNAs regulate fundamental processes such as cell proliferation and differentiation and that their expression is altered in some neoplasms. NCRNAs perform a considerable part of their function through interaction with proteins that regulate their biosynthesis and direct their function, but the characterization of these ribonucleoprotein complexes is far from complete. Modular expression or function of ncRNA in cancer cells can be an effective strategy to counteract the altered proliferation and invasiveness of neoplastic cells and the fundamental studies underway in our laboratory have this long-term goal.
The Facility, in which the two executives in service participate, studies the molecular regulation of messenger RNA metabolism and the function of non-coding RNA in mammal cells. The aim of our work is to understand how these processes can affect the pathogeneases of human diseases of wide social importance such as inflammation and cancer. We follow two main widely intersected lines of research:
Regulation of degradation of unstable transcribeds
The speed at which a messenger RNA (mRNA) is degraded is as important as the speed at which it is transcribed. Transcription and degradation determine the amount of each mRNA at any given time. Since the mRNAs coding for regulatory proteins (transcription factors, cyclones, oncogenes, cytokines, chemokines, etc.) are largely unstable, it is essential to understand the molecular mechanisms that a cell implements to regulate the rate of degradation of each mRNA in response to the variety of stimuli to which it is subjected during its lifetime. It is also known that transcription and degradation speeds are intimately connected. A key event in the degradation of an mRNA is the interaction of RNA-binding proteins (RBP) with specific sequences located in the un transcribed 3' (3' UTR) stroke of the transcribed. Once linked, these proteins recruit exoribonucleolytic enzymes responsible for mRNA degradation. The interaction with RNA and the functionality of RBPs is regulated by signaling pathways that convert the stimuli to which each cell is subjected in specific biochemical modifications of the RBS themselves. In addition, our laboratory recently proved that the interaction of a non-coding RNA (ncRNA, see point 2) with an RBP affects the pro-degrading function of the latter.
Regulation of gene expression by non-coding RNAs Only
a small part of the human genome (< 2%) coding for proteins. The remaining part, while not giving rise to proteins, does not constitute useless "junk", but can be transcribed and generate non-coding RNA (ncRNAs) that perform an important regulatory function on the expression of other genes. Hence the objective of identifying possible changes in expression or function at the expense of ncRNA in the course of diseases such as cancer. NCRNAs include both very short molecules (20-23 nucleotides, microRNAs [miRNAs]) and longer molecules (over 200 nucleotides, lncRNA). In the case of miRNAs, their pathogenic role in many human diseases is proven. In the case of lncRNAs, experimental research can be considered in the early days although it has recently been shown that some lncRNAs regulate fundamental processes such as cell proliferation and differentiation and that their expression is altered in some neoplasms. NCRNAs perform a considerable part of their function through interaction with proteins that regulate their biosynthesis and direct their function, but the characterization of these ribonucleoprotein complexes is far from complete. Modular expression or function of ncRNA in cancer cells can be an effective strategy to counteract the altered proliferation and invasiveness of neoplastic cells and the fundamental studies underway in our laboratory have this long-term goal.
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