基本信息
浏览量:11
职业迁徙
个人简介
Research Description
Fluorescent probes have revolutionized the study of biological macromolecules in their native environments. However, as compared to the extensive methodologies developed for proteins, relatively few imaging strategies are currently available for cellular nucleic acids. These methods are limited by their negative impact on native systems and/or inability to be applied in live cells and animals. Our group is therefore developing non-toxic fluorescent probes capable of characterizing the structure, function, and dynamics of nucleic acids in vivo. Daily work in our lab involves the design and synthesis of new fluorophores, nucleosides and oligonucleotides. In some cases, novel synthetic approaches are needed to obtain our target molecules. Next, extensive biological, photophysical and biophysical studies are used to characterize the candidates’ potentials to report nucleic acid structures and dynamics. Finally, promising compounds are evaluated in cell cultures and whole animals using fluorescence-based imaging. Some of the metabolic probes and methods developed in our lab have been commercialized by Sigma Aldrich and widely used by other research groups working in the fields of regenerative medicine, genome architecture, stem cell biology, and virology. Promising new applications of our probes include the development of diagnostic probes for personalized cancer therapy.
Fluorescent probes have revolutionized the study of biological macromolecules in their native environments. However, as compared to the extensive methodologies developed for proteins, relatively few imaging strategies are currently available for cellular nucleic acids. These methods are limited by their negative impact on native systems and/or inability to be applied in live cells and animals. Our group is therefore developing non-toxic fluorescent probes capable of characterizing the structure, function, and dynamics of nucleic acids in vivo. Daily work in our lab involves the design and synthesis of new fluorophores, nucleosides and oligonucleotides. In some cases, novel synthetic approaches are needed to obtain our target molecules. Next, extensive biological, photophysical and biophysical studies are used to characterize the candidates’ potentials to report nucleic acid structures and dynamics. Finally, promising compounds are evaluated in cell cultures and whole animals using fluorescence-based imaging. Some of the metabolic probes and methods developed in our lab have been commercialized by Sigma Aldrich and widely used by other research groups working in the fields of regenerative medicine, genome architecture, stem cell biology, and virology. Promising new applications of our probes include the development of diagnostic probes for personalized cancer therapy.
研究兴趣
论文共 77 篇作者统计合作学者相似作者
按年份排序按引用量排序主题筛选期刊级别筛选合作者筛选合作机构筛选
时间
引用量
主题
期刊级别
合作者
合作机构
CHEMICAL COMMUNICATIONSno. 9 (2024): 1156-1159
Bioconjugate chemistryno. 6 (2023): 977-982
BIOCONJUGATE CHEMISTRYno. 4 (2023): 638-644
Yizhu Li, Yurong Ling,Morten O Loehr, Sabrina Chaabane, Oh Wan Cheng, Kaifeng Zhao, Chao Wu, Moritz Büscher, Jana Weber,Daria Stomakhine,Marina Munker,Ronja Pientka,
Life sciences (2023): 122000-122000
Bioconjugate chemistryno. 6 (2023): 972-976
Bruktawit Maru, Alessandra Messikommer, Linhui Huang,Katja Seipel,Olivia Kovecses,Peter J M Valk,Alexandre P A Theocharides,Francois E Mercier,Thomas Pabst,Maureen McKeague,Nathan W Luedtke
Cell reports. Medicineno. 9 (2023): 101191
Alessandra Messikommer,Bruktawit Maru,Katja Seipel,Peter J. M. Valk,Alexandre P.A. Theocharides,Thomas Pabst,Maureen McKeague,Nathan W. Luedtke
biorxiv(2021)
加载更多
作者统计
合作学者
合作机构
D-Core
- 合作者
- 学生
- 导师
数据免责声明
页面数据均来自互联网公开来源、合作出版商和通过AI技术自动分析结果,我们不对页面数据的有效性、准确性、正确性、可靠性、完整性和及时性做出任何承诺和保证。若有疑问,可以通过电子邮件方式联系我们:report@aminer.cn