I have extensive training in physiology and neuropharmacology applied to visceral and somatic systems in the lower urinary tract. One of the most important discoveries involves my work on bladder and urethral dysfunction using animal models of lower urinary tract dysfunction induced by spinal cord injury, bladder outlet obstruction (BOO) and prostatic inflammation. Our findings revealed that chemical mediators such as growth factors and inflammatory changes in the lower urinary tract (bladder, urethra, prostate) significantly alter lower urinary tract function to induce bladder and urethral dysfunctions causing both storage and voiding problems, which potentially underlies lower urinary tract symptoms (LUTS). Thus, my expertise has laid the groundwork to study this new and exciting area exploring prostate-to-bladder sensitization mechanisms underlying bladder dysfunction and afferent hyperexcitability in chemically and genetically induced rodent models of prostatic inflammation. In summary, I have a demonstrated record of successful and productive research projects in this area of urologic problems, and my expertise and experience have prepared me to complete the proposed Project 2 of this U54 renewal application.