基本信息
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个人简介
Research Interests
The general theme of my research program is mechanotransduction by the blood and lymphatic vasculatures.
My recent research focuses on the mechanical and electrophysiological properties of lymphatic smooth muscle and endothelium and how their dysfunction contributes to lymphedema.
Currently funded as P.I. by two NIH R01 grants to study: 1) the ion channels involved in pacemaking of lymphatic muscle; and 2) the conduction of electrical signals along and across the lymphatic wall.
Serve as Co-I on three other NIH grants to contribute expertise in assessing mechanisms of lymphatic valve and contractile function in mouse models of human lymphatic disease, including lymphedema distichiasis, Cantu Syndrome and Ras-pathway mutations.
Areas of Expertise
My laboratory was one of the first to develop isolated vessel methods to study small arterioles, as well as patch clamp / calcium imaging methods to study mechanotransduction by single vascular cells.
We pioneered methods to study the mouse lymphatic system including:
1) the assessment of contractile and transport properties (contraction frequency, strength and transport rate) under controlled pressure / flow;
2) valve back-leak and leaflet closure tests at defined pressures;
3) membrane potential measurements in lymphatic muscle and lymphatic endothelium;
4) patch clamp characterization of specific ion channels in freshly isolated mouse lymphatic cells;
5) optogenetic techniques for recording Ca2+ signals in select cell populations of the lymphatic wall using GCaMP indicators
optogenetic techniques for manipulating cell signaling using genetically expressed channel rhodopsins.
We also study pressurized human lymphatic vessels, with which we seek to confirm the electrophysiology, contractile and valve properties identified in mice.
We have developed and maintain over 90 transgenic mouse models.
研究兴趣
论文共 341 篇作者统计合作学者相似作者
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JOURNAL OF PHYSIOLOGY-LONDON (2024)
Natureno. 7996 (2024): 1-10
Scott D Zawieja, Grace A Pea, Sarah E Broyhill, Advaya Patro, Karen H Bromert,Min Li,Charles E Norton,Jorge A Castorena-Gonzalez,Edward J Hancock,Christopher D Bertram,Michael J Davis
The Journal of general physiologyno. 12 (2023)
NATURE CARDIOVASCULAR RESEARCHno. 7 (2023): 702-702
Research squareno. 1 (2023): 15862-13
Function (Oxford, England)no. 3 (2023)
bioRxiv the preprint server for biology (2023)
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作者统计
#Papers: 341
#Citation: 14103
H-Index: 64
G-Index: 107
Sociability: 7
Diversity: 3
Activity: 84
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