The Rosengart Lab has a long-standing record of examining the role of innate immunity in the systemic response to injury and infection, with particular expertise in calcium-dependent mechanisms. The laboratory has focused upon the mechanisms involved in the inflammatory processes that define the response to injury in relevant sepsis and trauma models such as LPS-induced inflammation and organ dysfunction, cecal ligation and puncture (CLP) polymicrobial sepsis, warm hepatic ischemia/reperfusion (I/R) injury, and hemorrhagic shock. By employing diverse strategies such as in vivo siRNA to generate genetically engineered mice lacking key components of the calcium-sensitive CaMK cascade, high-speed depth resolved optical mapping of calcium transients, electron and fluorescent microscopy of subcellular organelle and protein trafficking, and study of the ramifications of calcium supplementation on inflammation and organ dysfunction, Dr. Rosengart has contributed to the understanding of calcium-dependent regulation and signaling during the inflammatory states of sepsis and injury. The translational perspective of his studies has provided mechanistic insight into the ramifications of the common practice of calcium supplementation in critical illness on the clinically relevant outcomes of organ failure and survival.