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We study epigenetic regulators using genetics, genomics and proteomics. The factors we study include the Polycomb and Trithorax Groups in both flies and humans, and chromatin-associated fusion oncoproteins such as BRD4-NUT and MOZ-TIF2 in human cancers. The common thread is that each is strongly implicated in the creation of active or silent chromatin domains that are integral to the fidelity of gene regulation. One serious obstacle to understanding the interactions of such factors with additional proteins and RNAs on chromatin has been the trade-off between removal from the DNA, to allow purification, and the resultant loss of interactions with key partners in function. Therefore, we have adapted a crosslinking approach that allows us to affinity-purify fragmented chromatin with protein and RNAs attached, to avoid disruption of interactions that may only occur on DNA. After reversal of crosslinks, the DNA, protein, histone peptides, and RNA fractions can be separately analyzed using comprehensive sequencing and mass spectrometry. Our current results are providing us with a rich and comprehensive view of key epigenetic complexes bound to their chromatin templates.
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Artyom A. Alekseyenko,Barry M. Zee, Zuzer Dhoondia, Hyuckjoon Kang, Jessica L. Makofske,Mitzi I. Kuroda
biorxiv(2023)
bioRxiv (Cold Spring Harbor Laboratory) (2022)
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Hyuckjoon Kang,Janel R Cabrera,Barry M Zee,Heather A Kang, Jenny Marie Jobe, Maeve B Hegarty,Aurelie E Barry,Alexander Glotov,Yuri B Schwartz,Mitzi I Kuroda
Hyuckjoon Kang,Janel R. Cabrera,Barry M. Zee, Heather A. Kang, Jenny Marie Jobe, Maeve B. Hegarty, Aurelie E. Barry,Alexander Glotov,Yuri B. Schwartz,Mitzi I. Kuroda
crossref(2022)
Kamila Naxerova,Bruno Di Stefano,Jessica L Makofske,Emma V Watson, Marit A de Kort,Timothy D Martin,Mohammed Dezfulian, Dominik Ricken,Eric C Wooten,Mitzi I Kuroda,Konrad Hochedlinger,Stephen J Elledge
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