基本信息
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职业迁徙
个人简介
The current project builds on my knowledge and technical expertise in molecular biology, immunology, genetics, genomics, GWAS, functional studies, and high-throughput screens. I had my Ph.D. training in molecular biology with Dr. Eric Hendrickson, focusing on human somatic cell gene targeting. My work was published in PNAS, 2003. I had my first postdoc training in molecular immunology with Dr. Frederick Alt, concentrated in generating murine model for human severe combined immunodeficiency disorders with a publication on Molecular Cell, 2008. The combination of these two training empowers me with very strong background in both molecular biology and immunology. For a family reason, I elected instead a staff scientist position in Dr. Robert Plenge’s lab after my training, working independently on functional analysis of GWAS hit. During this period of time, I acquired basic concept of human genetics, understood the power as well as the question, in particular, of GWAS analysis, developed a high throughput small molecule screen system to identify inhibitor for CD40 signal transduction, which is developed based on my functional analysis on a disease-associated CD40 locus. This work was published in PLos Genticis, 2013. Also, by collaborating with Dr. Gorge Church’s lab to identify rare variants from genes involved in rheumatoid arthritis by exon sequencing (exon-seq), with Dr. Stephen Elledge’s lab to identify autoantigens for juvenile idiopathic arthritis by phage immunoprecipitation sequencing (PhIP-seq), and with Broad Institute to analyze gene expression signature on CD40-activated B cells and monocytes by RNA sequencing (RNA-seq), I become an expert on genomics. In Sept. 2013, I decided to re-start my academic career in Dr. Nigrovic lab with the ultimate goal to apply human genetics and genomics to understand complex diseases such as autoimmune diseases and aging-related disease including cancers, cardiovascular diseases as well as neurodegenerative disorders by identifying and characterizing functional SNPs, and to develop allelic specific small molecule inhibitor to target the key transcription factors in disease-associated transcription regulation networks. Towards this end, I will focus on three key research areas: (1) Discovery genetics/genomics - use novel experimental high throughput screens to identify functional variants associated with complex diseases and traits, including disease susceptibility and response to therapy; (2) Mechanistic dissection - use novel molecular biology tools to understand the function of each functional variant by identifying the transcription factors involving effect gene expression; and build up a disease-associated transcription network. (3) Clinical translation - use genetics and mechanistic insight to help predict and/or prevent disease and to design high-throughput, small molecule drug screens to accelerate drug discovery. Within these five years, I have developed two novel techniques: functional SNP sequencing (fSNP-seq) and Franking Restriction Enzyme-mediated DNA Pulldown (FREP) to identify functional SNPs and characterize the mechanisms of functional SNPs as described in a publication in Nature Genetics (2018), which sets up the foundation for this project.
研究兴趣
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Aging Cellno. 2 (2023)
Lloyd D. Harvey, Hee-Jung J Kim,Mona Alotaibi, Yi-Yin Tai,Ying Tang,Sanya Arshad, Wei Sun,Haodi Wu,Jingsi Zhao,Anna Kirillova,Chen-shan J Woodcock,Adam Handen,
Circulationno. Suppl_1 (2022)
Circulation Researchno. Suppl_1 (2022)
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D-Core
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