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We are interested in the biogenesis and function of subcellular organelles. We have been concentrating on novel cytosolic ribonucleoprotein particles (RNPs) called vaults. Vaults were discovered in our laboratory and found to exist in most eukaryotic cells. They have an intricate shape composed of multiple arches reminiscent of cathedral vaults, hence their name. Vault size (~74 x 42 x42 nm), shape and localization suggests that they may involved in nucleo-cytoplasmic transport. We are interested in elucidating the function of these unique structures and in manipulating their structure to give them new functions. We are using the baculovirus expression system to produce recombinant vaults in order to test the concept that vaults can have a broad nanosystems application as malleable nanocapsules. Toward this aim we are currently designing particles with encapsulated fluorescent probes and enzymatically active protein domains. In addition, a number of strategies are currently being considered to encapsulate chemically active small molecules into the vault particle. If successful, these vault nanocapsules can be bioengineered to allow their use in a wide variety of biological applications including drug delivery, biological sensors, enzyme delivery, controlled release, and nano-electrical machine (NEMS) applications.
We are interested in the biogenesis and function of subcellular organelles. We have been concentrating on novel cytosolic ribonucleoprotein particles (RNPs) called vaults. Vaults were discovered in our laboratory and found to exist in most eukaryotic cells. They have an intricate shape composed of multiple arches reminiscent of cathedral vaults, hence their name. Vault size (~74 x 42 x42 nm), shape and localization suggests that they may involved in nucleo-cytoplasmic transport. We are interested in elucidating the function of these unique structures and in manipulating their structure to give them new functions. We are using the baculovirus expression system to produce recombinant vaults in order to test the concept that vaults can have a broad nanosystems application as malleable nanocapsules. Toward this aim we are currently designing particles with encapsulated fluorescent probes and enzymatically active protein domains. In addition, a number of strategies are currently being considered to encapsulate chemically active small molecules into the vault particle. If successful, these vault nanocapsules can be bioengineered to allow their use in a wide variety of biological applications including drug delivery, biological sensors, enzyme delivery, controlled release, and nano-electrical machine (NEMS) applications.
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论文共 40 篇作者统计合作学者相似作者
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Logan Thrasher Collins,Wandy Beatty, Buhle Moyo, Michele Alves-Bezerra,Ayrea Hurley, Qing Lou, Z. Hong Zhou,William Lagor,Gang Bao,Selvarangan Ponnazhagan, Randall McNally,Leonard H. Rome,
biorxiv(2024)
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