基本信息
views: 14
![](https://originalfileserver.aminer.cn/sys/aminer/icon/show-trajectory.png)
Bio
I contributed to the study of the involvement of Cyclophilin A (PPIA) in ALS pathology (Lauranzano et al., 2015). In particular, I generated a double transgenic mouse model of ALS (SOD1G93APPIA-/-) and I characterized it through biochemical, histological and behavioral analysis. I found that the absence of extracellular PPIA (ePPIA) functions are beneficial for ALS. I tested in vitro and in vivo inhibitors of ePPIA and found that MM218 is able to decrease the inflammatory response, protects motor neurons and increases survival in SOD1G93A mice (Pasetto et al., 2017). In parallel, I have been also characterizing PPIA-/- mice through longitudinal MRI, histopathological and biochemical analysis, motor and cognitive tests demonstrating that these mice display neuropathological alterations worsening with age, are defective in nucleocytoplasmic transport, TDP-43 autoregulation and synaptic function and develop cognitive, behavioral impairments and late-onset motor neuron disease (Pasetto L. et al., 2021). The analysis of PPIA-/- mice, together with the characterization of an ALS patient with a PPIA mutation, have highlighted that defective PPIA induces TDP-43 proteinopathy, suggesting important implications for ALS, FTD and others neurodegenerative diseases (Pasetto et al., 2021). Indeed, another study associated defective PPIA to RML-induced prion disease (Bouybayoune et al., 2019) and TBI.
I studied exosomes both as a new source of biomarkers for ALS and as mediator of toxicity (Basso et al., 2013; Ferrara D. et al., 2018; Pasetto et al., 2021), characterizing these vescicles through an array of omics technologies, imaging and biochemical assays. Our study conceived an innovative mathematical model based on machine learning which, by integrating EV size distribution data with protein cargoes, gave very high prediction rates for ALS disease diagnosis and prognosis (Pasetto et al., 2021). At the same time, I’m studying the role of EVs RNA in ALS pathogenesis and in patients profiling. In detail, I’m characterizing EVs derived from ALS primary astrocytes and I’m evaluating the effect of drugs able to inhibit RNA loading in EVs in the same ALS models.
Moreover, I contributed to biomarker research in ALS (Filareti et al., 2017; Crociare et al., 2018; Luotti et al., 2020; Beghi et al., 2022), I studied nitrated proteins in PBMC and spinal cord of two different strains of SOD1G93A mice (Nardo et al., 2016). Finally, I studied the potential of microtomography as a non-invasive technique for the evaluation of muscular atrophy in vivo in the SOD1G93A mouse model (Pasetto L. et al., 2018). I made my biochemical and immunoassay expertise available for different preclinical studies (Tortarola et al., 2015; Vallarola et al., 2018; Aquila et al., 2020; Vallarola et al., 2020).
Now, I am focusing on unveiling the molecular mechanism at the basis of TDP-43 pathology in two TDP-43 proteinopathies (FTD and TBI) and on characterizing the effects of PPIA delivered by a gene therapy approach on TDP-43 pathology, FTD and TBI (Cariplo foudation grant). I am evaluating the effect of different therapeutic approaches in the mouse model of ALS and TDP-43 proteinopathies. I am optimizing AlphaLISA and SIMOA technology for neurological biomarker research in tissue and biofluid for neurodegenerative diseases (ALS-FTD and TBI) and COVID-19 patients (Bonetto et al., 2022). During this period, I developed an expertise in in vivo technologies and behavioral analysis, proteomics, biochemistry, molecular biology, imaging, scientific writing, undergraduate/graduate students mentoring, laboratory training and scientific paper revision.
Research Interests
Papers共 26 篇Author StatisticsCo-AuthorSimilar Experts
By YearBy Citation主题筛选期刊级别筛选合作者筛选合作机构筛选
时间
引用量
主题
期刊级别
合作者
合作机构
Journal of Neuroinflammationno. 1 (2024): 1-11
Lucas Caldi Gomes,Sonja Hänzelmann,Fabian Hausmann,Robin Khatri,Sergio Oller, Mojan Parvaz,Laura Tzeplaeff,Laura Pasetto,Marie Gebelin, Melanie Ebbing, Constantin Holzapfel, Stefano Fabrizio Columbro,
bioRxiv (Cold Spring Harbor Laboratory) (2024)
Lucas Caldi Gomes,Sonja Hänzelmann,Fabian Hausmann,Robin Khatri,Sergio Oller, Mojan Parvaz,Laura Tzeplaeff,Laura Pasetto,Marie Gebelin, Melanie Ebbing, Constantin Holzapfel, Stefano Fabrizio Columbro,
Nature communicationsno. 1 (2024): 4893-4893
Valentina Kebede,Teresa Ravizza,Silvia Balosso,Rossella Di Sapia, Luca Canali,Sara Soldi,Serena Galletti,Christina Papazlatani,Panagiotis A. Karas,Sotirios Vasileiadis, Annalisa Sforzini,Laura Pasetto,
medRxiv (Cold Spring Harbor Laboratory) (2022)
Load More
Author Statistics
#Papers: 26
#Citation: 701
H-Index: 12
G-Index: 16
Sociability: 6
Diversity: 3
Activity: 25
Co-Author
Co-Institution
D-Core
- 合作者
- 学生
- 导师
Data Disclaimer
The page data are from open Internet sources, cooperative publishers and automatic analysis results through AI technology. We do not make any commitments and guarantees for the validity, accuracy, correctness, reliability, completeness and timeliness of the page data. If you have any questions, please contact us by email: report@aminer.cn