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职业迁徙
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In addition, in order to address the global shortage of donor tissues, were are currently involved in a study of corneal regeneration using tissue stem cells and induced pluripotent stem cells (iPS). In order to anatomically and functionally restore the cornea, our current studies involve the following: 1) Isolation of stem cells from the 3 layers of the cornea. 2) Induction of corneal cells from iPS cells. 3) Devising of tissue engineering techniques to produce 3-dimensional corneal tissue constructs. 4) Development of new surgical techniques. 5) Establishing the infrastructure required for the processing of safe tissue-engineered products.
Research activities
Isolation of mouse corneal epithelial stem cells
We devised a protocol using cholera toxin and serum-free medium to isolate corneal epithelial progenitor cells from a single mouse cornea (Ma X, Invest Ophthalmol Vis Sci 2009). One of the original cell lines has maintained a normal phenotype and karyotype through over 60 passages (Kawakita T. J Cell Mol Med. 2008).
Isolation of corneal stromal stem cells
We successfully isolated multipotent, neural crest-derived stem cells (COPs) from the adult murine cornea using the neurosphere technique (Yoshida S. Stem Cells 2006).
GMP grade cultivated epithelial sheets
We established a technique for engineering safe, transplantable epithelial sheets using donor human mesenchymal stem cells as feeder cells. The sheets are engineered in a cell processing center under strict sanitation standards. The initial clinical trial was approved by the Ministry of Health, Labour and Welfare.
Research activities
Isolation of mouse corneal epithelial stem cells
We devised a protocol using cholera toxin and serum-free medium to isolate corneal epithelial progenitor cells from a single mouse cornea (Ma X, Invest Ophthalmol Vis Sci 2009). One of the original cell lines has maintained a normal phenotype and karyotype through over 60 passages (Kawakita T. J Cell Mol Med. 2008).
Isolation of corneal stromal stem cells
We successfully isolated multipotent, neural crest-derived stem cells (COPs) from the adult murine cornea using the neurosphere technique (Yoshida S. Stem Cells 2006).
GMP grade cultivated epithelial sheets
We established a technique for engineering safe, transplantable epithelial sheets using donor human mesenchymal stem cells as feeder cells. The sheets are engineered in a cell processing center under strict sanitation standards. The initial clinical trial was approved by the Ministry of Health, Labour and Welfare.
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论文共 2213 篇作者统计合作学者相似作者
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Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologieno. 7 (2024): 2199-2207
Scientific reportsno. 1 (2024): 16129-16129
OCULAR SURFACE (2024): 139-144
Shinri Sato,Yoko Ogawa,Eisuke Shimizu,Kazuki Asai, Takahiro Okazaki, Robert Rusch,Masatoshi Hirayama,Shigeto Shimmura,Kazuno Negishi,Kazuo Tsubota
The Ocular Surface (2024): 198-210
The Ocular Surface (2024): 50-63
ENDOCRINE JOURNALno. 2 (2024): 153-169
Molecular Medicineno. 1 (2024): 1-11
Momoka Midorikawa,Kiwako Mori,Hidemasa Torii,Yohei Tomita,Yan Zhang,Kazuo Tsubota,Toshihide Kurihara,Kazuno Negishi
crossref(2024)
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