基本信息
浏览量:50
职业迁徙
个人简介
Overview
Multiplicity of Col11a1 function during development; structure and signaling
Extracellular matrix molecules play determining roles in development of complex structures such as the vertebrate eye and skeleton. Mutations in genes that encode proteins of the extracellular matrix demonstrate the importance of individual molecular constituents in the case of Stickler and Marshall syndromes in which a mutation in the Col11a1 gene causes both eye and bone manifestations in a given individual. The proposed work will address the hypothesis that Col11a1 acts to control cellular differentiation by interacting with regulatory signaling pathways. Alternatively, Col11a1 could act as a signaling molecule itself. Similarities exist between the phenotypes resulting from mutations in the BMP, Wnt, and PTHrP signaling pathways and mutations in Col11a1. The experimental approach proposed to address this hypothesis will combine three levels of analysis—a zebrafish model system for the analysis of vertebrate development, analysis of signaling pathways at the cellular level using cell culture, and molecular structure and interaction studies using biochemical and biophysical techniques to investigate molecular mechanisms of complex events during development and differentiation. The results of these studies will provide a more thorough understanding of the molecular and cellular mechanism of cell-matrix interactions and how such interactions influence signaling events. Moreover, we anticipate an increased understanding of how single molecules may be used for multiple purposes in a variety of contexts within a vertebrate organism, as well as the fundamental molecular events that are common to multiple unique outcomes during organogenesis. With an increase in understanding, we anticipate an improved ability to design new diagnostic approaches and therapeutic strategies to address the challenges introduced by mutations that affect eye and skeletal development.
Multiplicity of Col11a1 function during development; structure and signaling
Extracellular matrix molecules play determining roles in development of complex structures such as the vertebrate eye and skeleton. Mutations in genes that encode proteins of the extracellular matrix demonstrate the importance of individual molecular constituents in the case of Stickler and Marshall syndromes in which a mutation in the Col11a1 gene causes both eye and bone manifestations in a given individual. The proposed work will address the hypothesis that Col11a1 acts to control cellular differentiation by interacting with regulatory signaling pathways. Alternatively, Col11a1 could act as a signaling molecule itself. Similarities exist between the phenotypes resulting from mutations in the BMP, Wnt, and PTHrP signaling pathways and mutations in Col11a1. The experimental approach proposed to address this hypothesis will combine three levels of analysis—a zebrafish model system for the analysis of vertebrate development, analysis of signaling pathways at the cellular level using cell culture, and molecular structure and interaction studies using biochemical and biophysical techniques to investigate molecular mechanisms of complex events during development and differentiation. The results of these studies will provide a more thorough understanding of the molecular and cellular mechanism of cell-matrix interactions and how such interactions influence signaling events. Moreover, we anticipate an increased understanding of how single molecules may be used for multiple purposes in a variety of contexts within a vertebrate organism, as well as the fundamental molecular events that are common to multiple unique outcomes during organogenesis. With an increase in understanding, we anticipate an improved ability to design new diagnostic approaches and therapeutic strategies to address the challenges introduced by mutations that affect eye and skeletal development.
研究兴趣
论文共 119 篇作者统计合作学者相似作者
按年份排序按引用量排序主题筛选期刊级别筛选合作者筛选合作机构筛选
时间
引用量
主题
期刊级别
合作者
合作机构
BMC Research Notesno. 1 (2024): 1-2
Jennifer R Chase,Laura Bond, Daniel J Vail, Milan Sengthep, Adriana Rodriguez, Joe Christianson, Stephanie F Hudon,Julia Thom Oxford
COVIDno. 1 (2023): 23-37
International journal of molecular sciencesno. 6 (2023): 5526-5526
Lynn Karriem,Joshua Eixenberger,Stephanie Frahs,Domenica Convertino, Tyler Webb,Twinkle Pandhi, Kari McLaughlin,Ashton Enrriques,Paul Davis,Harish Subbaraman,Camilla Colletti,Julia T. Oxford,
bioRxiv : the preprint server for biology (2023)
Journal of biomolecular techniques : JBTno. 1 (2023): 3fc1f5fe.2f22458d-3fc1f5fe.2f22458d
Cameron Skaggs, Steve Nick,Conner Patricelli,Laura Bond,Kali Woods,Luke Woodbury,Julia Thom Oxford,Xinzhu Pu
BMC research notesno. 1 (2023): 1-7
Current protocolsno. 9 (2022)
加载更多
作者统计
合作学者
合作机构
D-Core
- 合作者
- 学生
- 导师
数据免责声明
页面数据均来自互联网公开来源、合作出版商和通过AI技术自动分析结果,我们不对页面数据的有效性、准确性、正确性、可靠性、完整性和及时性做出任何承诺和保证。若有疑问,可以通过电子邮件方式联系我们:report@aminer.cn