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Can we make cell reprogramming more efficient? Our group focuses on somatic cell nuclear transfer to amphibian eggs and oocytes from two complementary points of view. One aims to identify the molecules and mechanisms by which the cytoplasm of an egg or oocyte can reprogramme the nucleus of a differentiated somatic cell to behave like that of an embryo. From this state, many different kinds of cells for replacement can be generated.
The other aim is to identify the molecules and mechanisms that stabilise the differentiated state of somatic cells, as a result of which they resist reprogramming procedures. For these purposes we use single nuclear transfer to unfertilised eggs or multiple nuclear transfer to ovarian oocytes.
We make use of the special properties of an amphibian oocyte to inject messenger RNA that codes for a transcription factor protein. When this has been synthesised, it concentrates in the oocyte nucleus. The next day we inject plasmid DNA directly into the oocyte nucleus, where the factor causes transcription, and later expression, of a reporter gene in the plasmid.
The other aim is to identify the molecules and mechanisms that stabilise the differentiated state of somatic cells, as a result of which they resist reprogramming procedures. For these purposes we use single nuclear transfer to unfertilised eggs or multiple nuclear transfer to ovarian oocytes.
We make use of the special properties of an amphibian oocyte to inject messenger RNA that codes for a transcription factor protein. When this has been synthesised, it concentrates in the oocyte nucleus. The next day we inject plasmid DNA directly into the oocyte nucleus, where the factor causes transcription, and later expression, of a reporter gene in the plasmid.
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论文共 325 篇作者统计合作学者相似作者
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Cold Spring Harbor protocolsno. 12 (2021)
Mami Oikawa,Angela Simeone,Eva Hormanseder,Marta Teperek,Vincent Gaggioli,Alan O'Doherty, Emma Falk, Matthieu Sporniak,Clive D'Santos,Valar Nila Roamio Franklin,Kamal Kishore,Charles R. Bradshaw,
Experimental cell researchno. 2 (2020): 112296-112296
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#Papers: 271
#Citation: 23839
H-Index: 88
G-Index: 151
Sociability: 6
Diversity: 1
Activity: 0
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