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职业迁徙
个人简介
Primary Research Focus
The primary interest of the Payne Lab is to understand how excitable cells maintain intracellular ion and water homeostasis. We are particularly interested in the regulation of intracellular [Cl-] of neurons and cardiomyocytes.
Research Interests
My laboratory examines the cellular physiology of the cation chloride cotransport proteins, including the Na-K-Cl cotransporter (NKCC) and K-Cl cotransporter (KCC). We are particularly interested in the function of these transport proteins in excitable cells, i.e, neurons and muscle cells. We have identified an isoform of the K-Cl cotransporter (KCC2) that is important in maintaining low intracellular [Cl-] for the proper function of ligand-gated anion channels (GABAA and glycine receptors) in postsynaptic inhibition of mature neurons. The regulation of the cation chloride cotransporters is an important area of our research. Both NKCC and KCC2 appear to have significant roles in regulating intracellular [Cl-] and cell volume of neurons. How are changes in these cell parameters linked to alteration in the activity of the cotransporters? In the life of a neuron intracellular [Cl-] undergoes dynamic changes, such as during neuronal development or following traumatic insults. These changes in intracellular [Cl-] will lead to alterations in GABAAergic transmission. Are changes NKCC and KCC2 transport activity linked to these events and if so how? We are studying the regulation of NKCC and KCC2 by examining changes in both their kinetic activity and surface protein expression. In addition to their function at the cell level, I am interested in the structure of these proteins and how they function at the molecular level. How do the cotransporters interact with the ions they transport and the inhibitors they bind? Where are these sites of interaction within the protein? How do the cotransporters interact with other regulatory proteins? My laboratory uses whole animal, cellular, and molecular experimentation to answer many of these questions.
The primary interest of the Payne Lab is to understand how excitable cells maintain intracellular ion and water homeostasis. We are particularly interested in the regulation of intracellular [Cl-] of neurons and cardiomyocytes.
Research Interests
My laboratory examines the cellular physiology of the cation chloride cotransport proteins, including the Na-K-Cl cotransporter (NKCC) and K-Cl cotransporter (KCC). We are particularly interested in the function of these transport proteins in excitable cells, i.e, neurons and muscle cells. We have identified an isoform of the K-Cl cotransporter (KCC2) that is important in maintaining low intracellular [Cl-] for the proper function of ligand-gated anion channels (GABAA and glycine receptors) in postsynaptic inhibition of mature neurons. The regulation of the cation chloride cotransporters is an important area of our research. Both NKCC and KCC2 appear to have significant roles in regulating intracellular [Cl-] and cell volume of neurons. How are changes in these cell parameters linked to alteration in the activity of the cotransporters? In the life of a neuron intracellular [Cl-] undergoes dynamic changes, such as during neuronal development or following traumatic insults. These changes in intracellular [Cl-] will lead to alterations in GABAAergic transmission. Are changes NKCC and KCC2 transport activity linked to these events and if so how? We are studying the regulation of NKCC and KCC2 by examining changes in both their kinetic activity and surface protein expression. In addition to their function at the cell level, I am interested in the structure of these proteins and how they function at the molecular level. How do the cotransporters interact with the ions they transport and the inhibitors they bind? Where are these sites of interaction within the protein? How do the cotransporters interact with other regulatory proteins? My laboratory uses whole animal, cellular, and molecular experimentation to answer many of these questions.
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Naomi Fearon,Elizabeth Ryan,Gavin Stewart,Des Winter,Alan Baird, Leonard Quinn, K. Knight, R. Oliphant, F. Maxwell, C. McKenzie, T. Sharma, A. Macdonald,
semanticscholar(2014)
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