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Pseudomonas aeruginosa (P. aeruginosa) lung infection is a major cause of morbidity and mortality in cystic fibrosis (CF) patients. P.aeruginosa usually spreads to patients from the environment, but epidemic clones spreading patient-to-patient are an emerging threat to CF patients internationally. Dr Manos has characterised the molecular basis for infectivity of the epidemic clones AES-1 and AES-2 that infect up to 45% of CF patients in eastern Australia, by identifying expression and presence virulence factors specific to AES-1. The findingsare being used to develop novel therapeutics and infection control strategies, particularly with regard to biofilm disruption.
To further investigate CF-strain specific genes of P. aeruginosa, the CF research group at the University of Sydney, with Associate Professor Manos as a chief investigator, sequenced the genome of AES-1 and in conjuction with the Victorian Bioinformatics Institute (Monash University) developed a non-redundant array (PANarray) containing all genes from eight sequenced P. aeruginosa genomes. Dr Manos' lab used this array, together with an artificial sputum medium (ASMDM) that mimics CF sputum, to investigate expression of CF-strain specific genes. His lab created mutants in six genes significantly differentially expressed in ASMDM, and is investigating changes in virulence, infectivity and invasiveness in the mutants in the A549 epithelial cell line and the C57Bl/6J mouse model of lung infection (PloS one 2015).
The significance of the finding that better biofilm formation enhances virulence is being exploited in a number of pathogenic species that form biofilms. In 2018 Associate Professor Manos and Whiteley Corporation were successful in obtaining a $4.152 million research innovation grant through the iMCRC to investigate biofilm disruption in pathogenic bacteria. Thecurrent work with Postdoctoral Fellow Dr Theerthankar Das, (a recipient of a Sydney University Postdoctoral Fellowship), Whiteley Corporation scientists and his postgraduate students, involves investigating the effect of antioxidants on the structure of bacterial biofilms (AAC 2016, Frontiers in Microbiology 2017 and 2019, and JAC 2020).
Pseudomonas aeruginosa (P. aeruginosa) lung infection is a major cause of morbidity and mortality in cystic fibrosis (CF) patients. P.aeruginosa usually spreads to patients from the environment, but epidemic clones spreading patient-to-patient are an emerging threat to CF patients internationally. Dr Manos has characterised the molecular basis for infectivity of the epidemic clones AES-1 and AES-2 that infect up to 45% of CF patients in eastern Australia, by identifying expression and presence virulence factors specific to AES-1. The findingsare being used to develop novel therapeutics and infection control strategies, particularly with regard to biofilm disruption.
To further investigate CF-strain specific genes of P. aeruginosa, the CF research group at the University of Sydney, with Associate Professor Manos as a chief investigator, sequenced the genome of AES-1 and in conjuction with the Victorian Bioinformatics Institute (Monash University) developed a non-redundant array (PANarray) containing all genes from eight sequenced P. aeruginosa genomes. Dr Manos' lab used this array, together with an artificial sputum medium (ASMDM) that mimics CF sputum, to investigate expression of CF-strain specific genes. His lab created mutants in six genes significantly differentially expressed in ASMDM, and is investigating changes in virulence, infectivity and invasiveness in the mutants in the A549 epithelial cell line and the C57Bl/6J mouse model of lung infection (PloS one 2015).
The significance of the finding that better biofilm formation enhances virulence is being exploited in a number of pathogenic species that form biofilms. In 2018 Associate Professor Manos and Whiteley Corporation were successful in obtaining a $4.152 million research innovation grant through the iMCRC to investigate biofilm disruption in pathogenic bacteria. Thecurrent work with Postdoctoral Fellow Dr Theerthankar Das, (a recipient of a Sydney University Postdoctoral Fellowship), Whiteley Corporation scientists and his postgraduate students, involves investigating the effect of antioxidants on the structure of bacterial biofilms (AAC 2016, Frontiers in Microbiology 2017 and 2019, and JAC 2020).
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Arthika Manoharan,Jessica Farrell, Vina R. Aldilla,Greg Whiteley, Erik Kriel,Trevor Glasbey,Naresh Kumar,Kate H. Moore,Jim Manos,Theerthankar Das
Frontiers in Cellular and Infection Microbiology (2023): 1216798-1216798
ACS MATERIALS AUno. 4 (2023): 310-320
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