Currently, we are particularly interested in signaling pathways mediated by inositol phospholipid PtdIns(3,4,5)P3, inositol phosphates (e.g. InsP4 and InsP7), reactive oxygen species (ROS), and neutrophil serine proteinases (elastase, proteinase 3, and cathepsin G). We also identified gasdermin family proteins, particularly GSDMD and GSDME, as key cellular factors controlling neutrophil death and function. We utilize a wide variety of approaches ranging from basic molecular, cellular, and biochemical methods to automatic high-resolution imaging, scRNA-seq, high throughput chemical genetic screening, and animal inflammation models to dissect these pathways. The ultimate goal is to develop more efficient and effective therapies for treating various infectious and inflammatory diseases.