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个人简介
Giuseppe Vicidomini studied computer science at the Department of Computer and Information Science, University of Genoa (Italy) and received his Diploma cum laude in 2003 (advised by Prof. Mario Bertero and Prof. Patrizia Boccacci). From 2003 to 2007 he worked at the Laboratory of Advance Microscopy and Spectroscopy (LAMBS, University of Genoa, Italy); where he received his Ph.D (advised by Prof. Alberto Diaspro) about image processing and analysis for fluorescence microscopy.
From 2008 to 2011 he worked as a post-doctoral fellow at the Department of NanoBiophotonics (headed by Prof. Stefan W. Hell, Nobel Laureate in 2014), Max Planck Institute for Biophysical Chemistry (MPI, Germany); where he developed a new approach, based on the temporal analysis of the fluorescence signal, which allows stimulated emission deletion (STED) microscopy to achieve tens of nanometres spatial resolution with a substantial reduction of the dose of light requested, thus opening the effective application of STED microscopy with fluorescent proteins and living cells [Vicidomini 2011]. Currently, all commercially available STED microscopes implement this method - called gated-STED. This work pioneered the use of the temporal information channel of a microscope, such as the ability of measuring the fluorescence signal dynamics at the nanoseconds scale, for improving the microscope spatial-resolution \cite{Lanzano2015}.
Since May 2011 he works in the Department of NanoPhysics (headed by Prof. Alberto Diaspro), Italian Institute of Technology (IIT, Italy), where in September 2013 he obtained a Researcher position and in May 2016 he became principal investigator (Tenure Track, Stage II) of the Molecular Microscopy and Spectroscopy research line. Vicidomini is also Adjunct Professor at the University of Genoa. His research interests are primarily concentrated on theoretical design, development and validation of novel optical and analytical tools that allow the modern biologists to peer inside living biological systems with unprecedented temporal/spatial abilities and massive information content. While constantly working on STED microscopy [Vicidomini 2018], and on its combination with fluorescence-correlation spectroscopy (STED-FCS) [Vicidomini 2015, Lanzano2018], Vicidomini started also developing novel single-photon-avalanche diode (SPAD) arrays for fluorescence microscopy. This research has recently evolved in the invention of a new scanning microscopy technique able to double the spatial resolution of conventional microscopy, while maintain live-cell, multi-color, and three-dimension imaging capabilities and adding time-resolved spectroscopy/imaging (e.g., fluorescence lifetime, intensity fluctuation analysis, anti-bunching) [Castello 2019]. This technique has introduced a new paradigm in fluorescence microscopy which unlocks the secret carried by each single-photon, thus moving from the era of single-molecule microscopy to the era of single-photon microscopy.
Giuseppe Vicidomini work has been supported by grants from various agencies such as the Fondazione San Paolo (Italy), the Marie Sklodowska Curie Actions (MSCA) and the European Research Council (ERC). His most recent research grant is the five-years Consolidator Grant project, entitled “BrightEyes: Multi-Parameter Live-Cell Observation of Biomolecular Processes with Single-Photon Detector Array”. The overall objective of the BrightEyes project is to develop a set of innovative and non-invasive imaging and spectroscopy tools able to observe a single-biomolecule at work in a living multi-cellular system. Specifically, by exploring novel SPAD arrays, the BrightEyes project will implement an optical system able to continuously (i) track in real-time a biomolecule of interest; (ii) measure its nano-environment and its structural changes; (iii) observe its interactions with other biomolecules; (iv) visualize its sub-cellular micro-environment with nanometre resolution.
Giuseppe Vicidomini is currently involved as co-founder and scientific advisor in the realization of the Genoa Instruments spin-off company. The spin-off will be dedicated to launch a software and hardware add-on system which will transform any conventional confocal microscope into a high-resolution single-photon microscope.
Giuseppe Vicidomini acts as a reviewer for different founding agencies and various journals, including Nature Communications, Nature Methods, Nature Photonics, Scientific Reports, Optics Express, Optics Letters, Biomedical Optics Express, Optica, Methods, Journal of Microscopy, Microscopy Research and Techniques, and Nanoscale. He is co-editor of two special issues: “Fluorescence Microscopy in the Spotlight” for Microscopy Research and Techniques (with Dr. F. Cella Zanacchi and Dr. P. Bianchini), and “STED Microscopy” for Journal of Physics D (with Prof. C. Eggeling).
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Gaia Di Timoteo,Andrea Giuliani,Adriano Setti, Martina C Biagi,Michela Lisi,Tiziana Santini, Alessia Grandioso,Davide Mariani,Francesco Castagnetti,Eleonora Perego,Sabrina Zappone,Serena Lattante,
Nature communicationsno. 1 (2024): 5033-5033
Adaptive Optics and Wavefront Control for Biological Systems X (2024)
Single Molecule Spectroscopy and Superresolution Imaging XVII (2024)
Multiphoton Microscopy in the Biomedical Sciences XXIV (2024)
bioRxiv (Cold Spring Harbor Laboratory) (2024)
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI (2024)
bioRxiv (Cold Spring Harbor Laboratory) (2023)
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#Papers: 191
#Citation: 4282
H-Index: 32
G-Index: 63
Sociability: 6
Diversity: 0
Activity: 1
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