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个人简介
Gonçalo José Martins Afonso started his scientific studies in the BSc in Biochemistry at the University of Coimbra, Portugal, concluded in 2018, having developed a project about Rebaudioside A, a steviol glycoside obtained from the plant Stevia. Its effects in Gonçalo José Martins Afonso is a Biochemistry researcher and PhD student in Experimental Biology and Biomedicine at the University of Coimbra, Portugal. He began his scientific studies with a Bachelor of Science in Biochemistry, which he completed in 2018. During his undergraduate studies, he conducted research on Rebaudioside A, a steviol glycoside obtained from the plant Stevia. His project focused on analyzing its effects in oxidative stress and metabolism in the CA3 area of the hippocampus of murine brain slices. After completing his Bachelor's degree, he was invited to continue this study, allowing him to conclude and publish his findings.
In 2020, Gonçalo completed his Master's Degree in Biochemistry at the same university. His thesis focused on the impact of Rebaudioside A in the metabolism of human dermal fibroblasts and breast cancer cell lines Hs578t and MCF7, with a focus on its potential use in chemotherapeutic strategies. He also studied the impact
of different culture media on the metabolic phenotype of human fibroblasts to increase the physiological significance of those models. During his Master's degree, he was awarded a research grant under the initiative "Summer Course in Interdisciplinary Research, Development and Innovation in Cellular and Molecular
Metabolism" funded by FCT.
Currently, Gonçalo is continuing his research as a PhD student in PDBEB (PHD Programme in Experimental Biology and Biomedicine) of the Interdisciplinary Research Institute from the University of Coimbra (IIIUC). His current project regards the metabolic profiling and reversion of mitochondrial dysfunction in Amyotrophic lateral sclerosis patients' neuronal cells differentiated from urine-derived stem cells. He has been actively involved in research since 2017 and has a special interest in mitochondrial bioenergetics, oxidative stress, the development and validation of new experimental models, and novel therapeutic strategies.
Gonçalo has experience in using different methods and equipment due to the work developed over the years, such as cell culture methodologies, cell viability assays (e.g., resazurin, SRB), metabolic profiling (e.g., Seahorse XFe96, metabolic phenotype microarrays (Biolog Mitoplates), NMR), fluorimetric assays (e.g., H2DCFDA, autofluorescence), PCR, flow cytometry, bioinformatics, data analysis, and others.
During his academic career, Gonçalo has published three papers in journals in the first quartile of its area, one book chapter, as well as two oral presentations in international conferences with corresponding abstracts, one poster presentation in a national congress with abstract published, and another poster presentation at a
national event. All those works, except two of the papers, were as the first author.
Overall, Gonçalo is a dedicated and experienced Biochemistry researcher with a proven track record of successful research and publications. He is passionate about advancing the field and exploring new avenues for novel therapies and models.
研究兴趣
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Mitochondrial Intoxicationpp.101-131, (2023)
Carla Cavaleiro,Goncalo J. M. Afonso, Paulo J. Oliveira,Jorge Valero,Sandra I. Mota,Elisabete Ferreiro
FRONTIERS IN MOLECULAR NEUROSCIENCE (2023): 1229728-1229728
bioRxivno. 17 (2022): 5198-5217
crossref(2022)
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