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个人简介
As a Master student, I participated in the development and implementation of in vitro methodologies to assess the viability of Plasmodium falciparum resistant to artemisinin and derivatives, based on fluorescence assays. I used these methodologies to screen new compounds with anti-malarial activity.
My work during this period was intense, as demonstrated by the number of publications that came out of my research activity. The tasks I carried out included:
1) Development of fluorimetric assays capable to detect and quantify cellular viability
2) Optimization of an alternative protocol to the ring-stage survival assay of 0–3 h parasites
3) Evaluation of the morphology and degree of development of the parasites by light microscopy.
4) Evaluation of the development-nuclear division of the parasites by quantifying the intensity of fluorescence emitted by SYBR Green I.
5) Mitochondrial viability and localization with the fluorophore JC-1 and fluorescence microscopy.
6) Evaluation of the functional integrity of the mitochondria by quantifying the fluorescence intensity of the cationic dye Rh123 and their location using fluorescent microscopy.
7) Assessment of cell viability by means of the resorufin assay.
This research activity aroused my interest in the genetic basis of cell death and the role of mitochondria in this process. I also wanted to expand my technical spectrum towards molecular biology, and learn cloning procedures, genetics and protein chemistry.
研究兴趣
论文共 13 篇作者统计合作学者相似作者
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Susana G Martins,Vanessa Ribeiro, Catarina Melo, Cláudia Paulino-Cavaco,Dario Antonini,Sharadha Dayalan Naidu,Fernanda Murtinheira, Inês Fonseca, Bérénice Saget, Mafalda Pita, Diogo R Fernandes, Pedro G dos Santos,
biorxiv(2024)
Fernanda Murtinheira, Ana Sofia Boasinha, João Belo, Luana Macedo, Elisa Farsetti,Tiago T. Robalo,Vukosava M. Torres, Francisco R. Pinto,Adelaide Fernandes, Patricia Nascimento,Mario S. Rodrigues,Federico Herrera
crossref(2024)
INORGANIC CHEMISTRYno. 40 (2023): 16412-16425
F. Murtinheira, C. Duarte-Olivenza, M. Migueis,R. Letra-Vilela,V. Martin,C. Rodriguez,C. Valente, A. Oliva,F. Herrera
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PURE AND APPLIED CHEMISTRYno. 6 (2017): 765-780
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