Dhouha Daassi

Medical oncology, Immune-Oncologist, cancer immunology, Immune checkpoint blockade -Immune cell markers expression check in tumor cell lines namely T and B cells, monocytes/macrophages and DC -culturing several human and mouse tumor cell lines and maintaining them -multichannel FACS analysis -mouse tumor harvesting -mouse immunotherapy: MC38 colorectal tumor cell line injection to mice, after 7 days from tumor growth, measuring tumor size (survival curve and tumor size curve) -Pharmacodynamics: after tumor growth, starting the treatment by immune checkpoint blockade protein combination and monitoring the tumor growth size and mice status -Looking at HHLA2 as novel immune checkpoint :human tumor cell line phenotypic and finding the best candidates for HHLA2 expression