基本信息
浏览量:23
![](https://originalfileserver.aminer.cn/sys/aminer/icon/show-trajectory.png)
个人简介
I have experience in protein purification (Affinity, Ion Exchange and Size Exclusion Chromatography), culture of parasites (Toxoplasma gondii), cell tissue culture, antibody production (polyclonal and monoclonal) and proteomics analysis in order to study the role of venomous animal toxins. During these years I worked (graduate, master and PhD degree) in a laboratory that was focused on the purification and biochemical characterization of toxins. This work was important to understand the tissue injuries and inflammatory response caused by toxins during snake envenomation. Also, these toxins showed potential application as antitumoral, antiparasitic and bactericide. During my master’s project I performed protein purification and biochemical characterization of a fibrinogenolytic metalloproteinase (BpMPI) isolated from Bothrops pauloensis snake venom. Our work suggested that BpMPI function is associated with fibrinogen, glandular and tissue kallikrein substrate, leading to potential application in biotechnology research. My PhD degree was to evaluate the production of antibodies against BpMPI in chicken and mice. This work demonstrates the efficiency of these polyclonal antibodies to recognize metalloproteinases in different venoms, including proteins that are responsible for local hemorrhage, showing potential for antiophidic serum production. As a result of this project, chicken egg demonstrated to be a good alternative for antibodies production. This work involved a variety of biochemistry and immunology techniques such as proteomics approach (2D gels and mass spectrometry), western blotting analyses and cell culture. As a post-doctoral I am highly independent and able to perform several biochemistry and immunology techniques like SPOT synthesis, monoclonal antibodies production and animal experimentation (Intracerebral-Ventricular, Peritoneal and Intramuscular injection).
研究兴趣
论文共 23 篇作者统计合作学者相似作者
按年份排序按引用量排序主题筛选期刊级别筛选合作者筛选合作机构筛选
时间
引用量
主题
期刊级别
合作者
合作机构
Silvio Rincon-Filho,Dayane Lorena Naves-de-Souza,Leticia Lopes-de-Souza, Jamil Silvano-de-Oliveira,Cesar Bonilla Ferreyra,Fernanda Costal-Oliveira,Clara Guerra-Duarte,Carlos Chávez-Olórtegui
Lucas S de Faria,Dayane L N de Souza, Raphaella P Ribeiro,José Eduardo N de Sousa,Isabela P Borges, Veridiana M R Ávila,Álvaro Ferreira-Júnior,Luiz Ricardo Goulart,Julia M Costa-Cruz
加载更多
作者统计
合作学者
合作机构
D-Core
- 合作者
- 学生
- 导师
数据免责声明
页面数据均来自互联网公开来源、合作出版商和通过AI技术自动分析结果,我们不对页面数据的有效性、准确性、正确性、可靠性、完整性和及时性做出任何承诺和保证。若有疑问,可以通过电子邮件方式联系我们:report@aminer.cn